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參數(shù)資料
| 型號(hào)： | A701 |
| 廠商： | Electronic Theatre Controls, Inc. |
| 英文描述： | A701: Test slide, various cancers plus corresponding normal |
| 中文描述： | A701：測(cè)試幻燈片，各種癌癥加上相應(yīng)的正常 |
| 文件頁數(shù)： | 8/10頁 |
| 文件大小： | 217K |
| 代理商： | A701 |
Appendix: application protocol 1
Deparaffinizing and H&E stain
For research use only
Deparaffinization and hydration
Dry the slide at 58
℃
for 1hr or overnight
, before deparaffinization (put slides in horizontal position)
①
Xylene (removal of paraffin) 4 X 10 min
↓
②
100% Ethanol (de xylene)
95% Ethanol 1min
95% Ethanol 1min
80% Ethanol 1min
70% Ethanol 1min
↓
③
Wash (tap water) until washing is completed (5 min)
Routine H&E stain
①
Wash (tap water) until washing is completed (5 min)
↓
②
Hematoxylin (Harris,nucleus staining,over staining) 3 min
↓
③
Wash (tap water)
↓
④
Decolor in 0.1% HCl, 70% Ethanol : repetitive dipping
↓
⑤
Neutralization 10 min (top water 5min/ Ammonia water repetitive dipping)
↓
⑥
Eosin (cytoplasm staining) 1 min
↓
⑦
Washing 30 sec
↓
⑧
70% Ethanol 1 min
80% Ethanol 1 min
95% Ethanol 1 min
95% Ethanol 1 min
100% Ethanol 1 min
↓
⑨
Xylene (clear to increase refractive index to 1.5 fold) 4 X 10 min
↓
⑩
Mount with mounting solution ( eg. balsam)
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